graph TD A[Load scRNA-seq Data] --> B[Load Dupilumab Targets] B --> C[Extract Primary Targets] C --> D[Split Lesion/Normal] D --> E[Create UMAP Visualizations] E --> F[Plot Primary Target Expression] F --> G[Side-by-side Comparison] subgraph "Primary Targets" H[IL4/IL4R] I[IL13/IL13RA1/IL4R] J[TGFB1/TGFBR1/TGFBR2] K[IL6/IL6R/IL6ST] L[IL1B/IL1R1/IL1R2] M[TNF/TNFRSF1A/TNFRSF1B] end